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pTYB4载体

Product Introduction

品牌

酶研生物

货号

MY1891

规格

2ug

价格

询价

货期

现货
质粒类型: 大肠杆菌表达载体
载体大小: 7474 bp 
5' 测序引物: T7
备注: This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein.

订购信息

产品编号 产品名称 规格 价格
1891 pTYB4

2ug质粒

询价

质粒图谱

pTYB4 质粒图谱

载体描述

描述

pTYB4 is an E. coli expression vector (7,474 bp) used in the IMPACT™ Kit (NEB #E6901) (1). This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa) (1,2). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein (3).

特色

  • The NcoI site in the polylinker contains an ATG sequence for translation initiation.
  • Use of the SmaI site for cloning will yield a target protein with a single glycine residue added to its C-terminus after cleavage.
  • Unique sites indicated in bold.
  • Expression of the fusion gene is under the control of an IPTG-inducible T7 promoter (4).
  • Origin of DNA replication from bacteriophage M13, which allows for the production of single-stranded DNA by helper phage superinfection of cells bearing the plasmid (M13K07 Helper Phage, NEB #N0315)
  • A pBR322 derivative with a ColE1 derivative origin
  • Ampicillin resistance.
  • Other IMPACT vectors are available which allow for fusion of a target gene to N-or C- terminus of an intein. The cleavage reaction may be induced by thiol reagent or temperature/pH shift.
  • Companion vectors (pTYB1, pTYB2, pTYB3) differ only in the sites present in the polylinker.
  • A wide range of E. coli host strains: T7 Express Competent E. coli (High Efficiency) (NEB #C2566) or BL21(DE3) Competent E. coli (NEB #C2527); and derivatives.
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