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pMAL-p5X载体

Product Introduction

品牌

酶研生物

货号

MY2056

规格

2ug

价格

询价

货期

现货

基本信息

质粒类型: 大肠杆菌表达载体
表达水平:
启动子: Tac
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5752 bp 
载体抗性: Ampicillin (氨苄青霉素)

订购信息

产品编号 产品名称 规格 价格
MY2056 pMAL-p5X 2ug质粒 1000

质粒图谱

pMAL-p5X质粒图谱
pMAL-p5X多克隆位点

载体描述

The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa (NEB #P8010).

MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin.

A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.

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