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pQCXIN载体

Product Introduction

品牌

酶研生物

货号

MY1536

规格

2ug

价格

询价

货期

现货

基本信息

质粒类型: 逆转录病毒载体
启动子: CMV/MSV
载体大小: 7400 bp 
5' 测序引物及序列: QC: 5'd[ACGCCATCCACGCTGTTTTGACCT]3'
载体标签: Ampicillin (氨苄青霉素)
载体抗性: IRES-Neo
筛选标记: Neomycin (新霉素)

订购信息

产品编号 产品名称 规格 价格
MY1536 pQCXIN 2ug质粒 1000

质粒图谱

pQCXIN质粒图谱

载体描述

pQCXIN Retroviral Vector is a bicistronic expression vector designed to express a target gene along with the neomycin selection marker (1). Upon transfection into a packaging cell line, this vector can transiently express, or integrate and stably express a viral genomic transcript containing the CMV immediate early promoter, gene of interest, IRES and the neomycin resistance gene (Neor). The gene of interest and the neomycin resistance gene are co-translated, via the internal ribosome entry site (IRES), from a bicistronic message in mammalian cells (2, 3).

This vector incorporates unique features including: optimization to remove promoter interference and self-inactivation. The hybrid 5' LTR consists of the cytomegalovirus (CMV) type I enhancer and the mouse sarcoma virus (MSV) promoter. This construct drives high levels of transcription in HEK 293-based packaging cell lines due, in part, to the presence of adenoviral E1A (4, 5, 6, 7) in these cells. The self-inactivating feature of the vector is provided by a deletion in the 3' LTR enhancer region (U3). During reverse transcription of the retroviral RNA, the inactivated 3' LTR is copied and replaces the 5' LTR, resulting in inactivation of the 5' LTR CMV enhancer sequences. This may reduce the phenomenon known as promoter interference (8) and allow more efficient expression.

Also included in the viral genomic transcript are the necessary viral RNA processing elements including the LTRs, packaging signal (Psi+), and tRNA primer binding site. pQCXIN also contains a bacterial origin of replication and E. coli Ampr gene for propagation and selection in bacteria.

载体应用

pQCXIN is a self-inactivating retroviral vector that is optimized to give high titers, and minimize the possibility of promoter interference. Expresses a gene of interest with the neomycin resistance gene as a bicistronic transcript. pQCXIN is also supplied with the BD Retro-X™ Q Vector Set.

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