The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway® pcDNA™-DEST40 vector offers the following key features:

• C-terminal V5-6x His tag for easy detection and rapid purification
• Cytomegalovirus (CMV) promoter for high-level expression
• attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
• Neomycin resistance gene for stable selection
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli