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pET-30 Xa/LIC载体

Product Introduction

品牌

酶研生物

货号

MY1951

规格

2ug

价格

询价

货期

现货
质粒类型: 大肠杆菌表达载体
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5448 bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-His, N-S, N-Xa Factor, C-His
载体抗性: Kanamycin (卡那霉素)
备注: For directional cloning of PCR-amplified DNA; ligation independent cloning; Factor Xa cleavage site

订购信息

产品编号 产品名称 规格 价格
1951 pET-30 Xa/LIC

2ug质粒

询价

质粒图谱

pET-30 Xa/LIC 质粒图谱
pET-30 Xa/LIC 载体图谱

载体描述

The pET-30 Xa/LIC vector is designed for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides containing N-terminal His•Tag® and S•Tag™ sequences for detection and purification. Using specifically designed primers for amplification and the pET-30Xa/LIC Vector Kit (Cat. No. 69073-3), inserts can be efficiently cloned without the need for restriction digestion or ligation, and the resulting fusion proteins can be cleaved with Factor Xa to precisely remove all vector-encoded amino acids. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

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