品牌 |
酶研生物 | |||||||||||||||||||||||||
货号 |
MY1239 | |||||||||||||||||||||||||
规格 |
2ug | |||||||||||||||||||||||||
价格 |
询价 | |||||||||||||||||||||||||
货期 |
现货 | |||||||||||||||||||||||||
载体说明pCDFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.
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