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pEGFP-Actin载体

Product Introduction

品牌

酶研生物

货号

MY1128

规格

2ug

价格

询价

货期

现货
质粒类型: 荧光蛋白报告载体
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5820 bp 
载体抗性: Kanamycin (卡那霉素)
筛选标记: Neomycin (新霉素)

订购信息

产品编号 产品名称 规格 价格
1128 pEGFP-Actin

2ug质粒

询价

质粒图谱

pEGFP-Actin 质粒图谱

载体描述

pEGFP-Actin encodes a fusion protein consisting of the red-shifted, human codon-optimized variant of green fluorescent protein (EGFP; 1–3) and the gene encoding human cytoplasmic b-actin (4). EGFP, a derivative of the GFPmut1 variant (5), has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) This variant contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr, and also contains more than 190 silent base changes which correspond to human codon-usage preferences (6). SV40 polyadenylation signals downstream of the EGFP-Actin fusion direct proper processing of the 3' end of the EGFP mRNA. The vector backbone also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T-antigen. A neomycin resistance cassette (Neor), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV-TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli. The pEGFPActin backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

载体应用

The pEGFP-Actin Vector expresses the EGFP-Actin fusion protein in mammalian cells. The protein is incorporated into growing actin filaments and allows for visualization of actin-containing subcellular structures in living and fixed cells. (7, 8). pEGFP-Actin can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (9).

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