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pET-42b(+)载体

Product Introduction

品牌

酶研生物

货号

MY1228

规格

2ug

价格

询价

货期

现货
别名: pET42b, pet 42b, pET-42b(+)
质粒类型: 大肠杆菌表达载体
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5929bp 
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-GST, N-His, C-His
载体抗性: Kanamycin (卡那霉素)
备注:
含有GST融合蛋白;N端Thrombin蛋白酶切位点; 含有N端Factor Xa蛋白酶切位点 ; pET 42 a,b,c 的差异仅在多克隆位点区域。

订购信息

产品编号 产品名称 规格 价格
1228 pET-42b(+)

2ug质粒

询价

质粒图谱

pET-42b(+) 质粒图谱
pET-42b(+) Vector Map

载体描述

The pET-42 series is designed for cloning and high-level expression of peptide sequencesfused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encodedsequence can be completely removed when cloning into the PshAI site (as shown below) and thencleaving the GST fusion protein with Factor Xa.

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