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pLVTH载体

Product Introduction

品牌

酶研生物

货号

MY1411

规格

2ug

价格

询价

货期

现货
质粒类型: 慢病毒表达载体,RNAi载体
启动子: H1
载体大小: 11086 bp
载体抗性: Ampicillin (氨苄青霉素)

订购信息

产品编号 产品名称 规格 价格
1411 pLVTH

2ug质粒

询价

质粒图谱

pLVTH 质粒图谱

载体描述

载体说明

This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA. For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid). Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning. Article: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz M et al. (J Virol. 2003 Aug . 77(16):8957-61.

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