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pLVTHM载体

Product Introduction

品牌

酶研生物

货号

MY1412

规格

2ug

价格

询价

货期

现货
质粒类型: RNAi载体
启动子: H1
载体大小: 11085 bp 
载体抗性: Ampicillin (氨苄青霉素)

订购信息

产品编号 产品名称 规格 价格
1412 pLVTHM

2ug质粒

询价

质粒图谱

pLVTHM 质粒图谱

载体描述

pLVTHM allows for direct cloning of shRNAs into the lentiviral vectors and replaces the old pLVTH system from Trono lab. pLVTHM is similar to pLVTH, but contains a 3bp substitution that generates a unique MluI site for direct cloning of an shRNA into MluI-ClaI. Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation (this site does not appear in the depositor's full sequence). The plasmid needs to be grown in a Dam+ bacterial strain in order to use ClaI for cloning. Note that ClaI has lower salt concentration requirements than MluI. One way to handle this is to digest with ClaI for 45 minutes, followed by addition of diluted MluI buffer and MluI, then incubation for 90 more minutes. Be sure not to use a large amount of vector (1.7 ug to 2.5 ug is known to work). Also, if your shRNA is already in pSUPER (or another plasmid under the control of the PolIII promoter) you may use the EcoRI-ClaI sites to replace the H1 promoter in pLVTHM with the H1-shRNA cassette from pSUPER (or another plasmid).

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