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pMIR-REPORT Luciferase载体

Product Introduction

品牌

酶研生物

货号

MY1399

规格

2ug

价格

询价

货期

现货
质粒类型: RNAi载体
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6470 bp
5' 测序引物: Luc-F, M13-F20
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Puromycin (嘌呤霉素)

订购信息

产品编号 产品名称 规格 价格
1399 pMIR-REPORT Luciferase

2ug质粒

询价

质粒图谱

 Luciferase 质粒图谱

载体描述

The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.

  • Clone miRNA targets and evaluate miRNA regulation
  • Screen putative miRNA target sequences
  • Includes both reporter and control vectors

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT™ Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT™ Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

载体应用

pMIR-REPORT™ Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT™ Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR™ miRNA Inhibitor Molecules, respectively.

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