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pMXB10载体

Product Introduction

品牌

酶研生物

货号

MY1251

规格

2ug

价格

询价

货期

现货
质粒类型: 大肠杆菌表达载体,
启动子: T7
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7816bp 
载体抗性: Ampicillin (氨苄青霉素)

订购信息

产品编号 产品名称 规格 价格
1251 pMXB10

2ug质粒

询价

质粒图谱

pMXB10 质粒图谱

载体描述

pMXB10 is a control plasmid for the IMPACT Kit (NEB #E6901) (1,2). This plasmid carries the E. coli malE gene, encoding the maltose binding protein (MBP, 42 kDa) (3), fused in-frame to the coding region of the Mxe gyrase A intein-chitin binding domain (28 kDa) from pTXB1 (2,4,5). pMXB10 can be used to test plasmid transformation, cell culture, induction and purification procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours), 100 ml of cells should yield 2-3 mg of a 70 kDa fusion protein. After chitin column purification and cleavage, approximately 1.5-2.0 mg of MBP is usually obtained. This double stranded vector is 7813 bp in length.

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叶子 135-6489-5879


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