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pOG44载体

Product Introduction

品牌

酶研生物

货号

MY1310

规格

2ug

价格

询价

货期

现货
质粒类型: 哺乳细胞表达载体
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5785bp
载体抗性: Ampicillin (氨苄青霉素)

订购信息

产品编号 产品名称 规格 价格
1310 pOG44

2ug质粒

询价

质粒图谱

pOG44 质粒图谱

载体描述

载体说明

pOG44 is a 5.8 kb Flp recombinase expression vector designed for use with the Flp-In. System (Catalog nos. K6010-01 and K6010-02) available from Invitrogen. When cotransfected with the pcDNA5/FRT plasmid into a Flp-In. mammalian host cell line, the Flp recombinase expressed from pOG44 mediates integration of the pcDNA5/FRT vector containing the gene of interest into the genome via Flp Recombination Target (FRT) sites. The vector contains the following elements:

  • The human cytomegalovirus (CMV) immediate-early enhancer/promoter for highlevel constitutive expression of the Flp recombinase in a wide range of mammalian cells (Andersson et al., 1989; Boshart et al., 1985; Nelson et al., 1987)
  • Synthetic intron to enhance expression of the FLP gene (Huang and Gorman, 1990; O’Gorman et al., 1991)
  • FLP gene encoding the Flp recombinase (Buchholz et al., 1996) to mediate integration of the pcDNA5/FRT expression plasmid into the genome
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